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 Posted: Mon 16:28, 06 Dec 2010 Post subject: ugg boots günstig Produce acetic acid by immobili |
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Produce acetic acid by immobilized cells of factors
Continued reaction of acetic acid, so that the yield continued to increase. Second, whether it is aerobic, anaerobic, glucose can be in the role of metabolic enzymes to form acetic acid, from glucose figure shows the change over time: glucose is in a lot of consumption of between 72 ~ 96h. Certainly part of the glucose into acetic acid. Conclusions: considered from the actual industrial production, the fermentation time is usually maintained at 72h, the initial ethanol content of 5.09 / 6. 2.8 alcohol content is not at the same time the initial cell number variation with time when the Figure 10 to 12h Q intervals were measured ethanol content (volume fraction) was 4.5%, 5.0%, 6.5%, 7.0,[link widoczny dla zalogowanych], culture conditions for the 180r/rain, 30 ℃,[link widoczny dla zalogowanych], the bacteria at different time points the number of changes bacterial counts of 10 units / mI. Can be seen from Figure 10, the ethanol concentration change at any time asked the number of bacteria growing trend, 0 ~ 24h, asked basically had no growth of bacteria in paragraph, in the growth delay of the different ethanol lhl/h0 Figure l0 different ethanol Under the initial content of cell number with time Fig. 10Differentinitialconcentrationsofethanolcellnumberchangesovertime the same content on the inhibition of bacteria; in the 24 ~ 48h time period, the number of the fastest growing cell, a linear increase in the logarithmic phase; in ethanol fermentation after 48h of cell growth trend the number of go gently. Also can be seen: the initial concentration of ethanol under different, with the reaction,[link widoczny dla zalogowanych], gradually approaching the number of bacteria, indicating that the inhibitory effect of ethanol gradually disappear. 3 Conclusion of the production of acetic acid by immobilized cells and the kinetics of the optimization, access to the following conclusions: 1) Optimizing the number of 15 rings inoculation, culture, asked for the 36 ~ 48h, the number of maximum cell fermentation; 2) in the ethanol content in each, 5.0% ethanol concentration of the highest conversion rate of acetic acid fermentation, the best training time was 72h. In the 72h time point, adding the right amount of glucose and other medium components, cells can continue to grow, to extend cell fermentation period; if adding an appropriate amount of ethanol, can continue to acetaldehyde into ethanol to produce acetic acid, can improve the yield. References: [1] Wu Tianrong, Cao Boxing, Xu Li, foreign chemical snowmelt development and progress of de-icing agent [J]. Salt industry, l989 (5); 28-33, [2] Xiao Jinbing, Xing Jun, SUN Yong-positive. CMA Preparation and melting snow and ice melting ice effect of small scale EJ]. Dalian University of Technology, 2003,24 (4) :32-34. [3] Bao Min, WANG long term Zheng Yue. Type of environmental de-icing agent [J]. Low Temperature Architecture Technology, 2002 (3): 3l a 32. [43sANTAGATAMC,[link widoczny dla zalogowanych], COLLEPARDIM. TheeffectofCMAdeicersonconcreteproperties [J]. CementandConcreteResearch, 2000,30 (9): l389 a l394. [5] ROBI13 () UX1PY, DELISLECE. EcotoxicologicalEvaluationofThreeDeicers (NaCl, NaFo, CMA) of a EffectonTerrestrial [J]. EffectonTerrestrial () rgan-ismsEcotoxicologyandEnvironmentalSafety, 200l, 48 (2): l28 a l39. [6] TAOGuoliang, zHUORenxi, CHENZhenhua. Study () nImmobilizedThermolysinWithCelluloseasCarrier [J]. ChineseJournalofReactivePolymers, 1996,5 (Bu 2): l9-25. [7] Chen Jianbin, Director of Red Star. Research on environment-friendly road deicing salt [J]. Chemical Engineer, 2004,10 (2): 1002 A l124. [8] Zhu Newport. New clean snow / ice Synthesis and Evaluation of [J]. Chemical Industry and Engineering, 2002,23 (1) :6-8. [9] New Li Ji, Zhang Chao,[link widoczny dla zalogowanych], Gao Hong. Application of Immobilized Cell Technology Research [J]. Chemical and Biological Engineering, 2006,23 (6) :5-7.
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