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PostPosted: Fri 20:02, 03 Dec 2010    Post subject: ugg boots günstig Bacillus subtilis Ki-2-132 high

Bacillus subtilis Ki-2-132 high expression of genetic engineering systems


Variants, this mutation accompanied by high-cycloserine resistant mutant (13O/m1). It showed well for the ability AprE secretion and expression, but also on the expression vector pkpl very stable, but, and de/s32 (Hly) and pM441 (dcgR) coordination of the effect is not good (basically Ki ~ 2-132 in similar)】, which also proved from the other side constructed using multi-effect regulation of gene expression of bacteria Bacillus subtilis drunk a 2-132 system genes must be coordinated growth and differentiation of views and ideas. IV Conclusion and more efficient use of regulatory genes and competent, conidial coordination among the relevant genes to reconstruct a 2-132 Bacillus subtilis Ki 5O growth and differentiation of a Pan Xuefeng et al: High expression of Bacillus subtilis Ki a 2132 study patterns of genetic engineering systems to gain a high expression AprE genetic engineering system. Development and use of this work to the safety of Bacillus subtilis can secrete the advantages of becoming a reality, and the national economy and for the further study of the prokaryotic and eukaryotic protein highly expressed genes laid the foundation for genetic engineering. 1989 References: [1: Wuzhou Xuefeng, Tang Mao-Qiang. Summary of Proceedings microbial genetics, Shengzu Jia, Chen Yongqing editor. Fudan University Press. Shanghai ,1994,91-102 [23MoniatisTeta1. Mo] ecu] arClon [rlg: ALaboratoryManua1. ColdSpringHarborLaboratoryPress, NewYorkL3] ChangSeta1. , Md. . Ge ~ e1.1g79.1fi8: 11l [43 Pan Xuefeng. Dissertation Chinese Academy of Sciences. Institute of Genetics, Chinese Academy of Sciences, 1993 [5] Microbial mutation breeding,[link widoczny dla zalogowanych], Science Press, 1979 [6] Pan Xuefeng. Beijing University Press, Research Institute of Genetics, Chinese Academy of Sciences Annual Report. 1992.12; 13 [7 Pan Xuefeng and so on. Microbiology, 1995 [8] PanXuefengeta1. trod mouth. . . A rising sun. 1988: l023 [93 Pan Xuefeng and so on. Genetics, 1995C103 Pan Xuefeng and so on. 9 China International Biotechnology and Pharmaceutical Industry Symposium ,1995,66-78 [】 1] Pan Xuefeng, ZHANG Yin Mei, Tang Mao station. Science Bulletin. i995TheStudyonHigh-expressiveGeneticalEngineeringSystemsinBacillusSubtilisKi a 2 a l32 (receivedMay20,[link widoczny dla zalogowanych], 1995, revisedJuly31) PanXuefeng, ZhangYinmei, TangMaohong (InstituteofGenetics, AcademiaSinica, Beijing1O010I) AI ~ l / 'actUsingthecooperationetweensperulationandcompetence-formingrelatedgeneothfromotherpleitropicregulatorygenesofBacillus, hpr, degQa,[link widoczny dla zalogowanych], degU/S32 (Hy) anddegRandfromtheBacillussubtilisKi a 2-132 . Theserieshish-expressivegetteticalengineerthghoststrainswereconstructed. The ~ estrainscouldexpresstheAprEataquithighleve1. Amongthem,[link widoczny dla zalogowanych], thestrainwhichconstructedbythecooperationofhpr, cornandspoere. , HadthecapacityforAprE-producingby1O0-200foldhig} 1el \Keywords: BacillussubtilisKi a 2-132,[link widoczny dla zalogowanych], Regulatorygone, AprE5l a

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