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 Posted: Mon 14:45, 28 Feb 2011 Post subject: timberland scarpe Erigeron Injection Extraction _5 |
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Erigeron Injection Extraction
From the 70 years since the great development of injection developed fleabane, Erigeron capsule, film Yi Mai Kang, breviscapine, films and other preparations Breviscapine I species for clinical use. Erigeron Injection preparation of 70 to 80 years, the basic use ever since, was limited to the technical standards and requirements, many indicators of relatively extensive, in order to further improve and standardize preparation process, improve the utilization of medicinal materials, product quality control , we conducted a study for the extraction process. 1 apparatus, reagents, materials, UV-visible spectrophotometer 7520. Al (NO), NaNO, and other test reagents were of analytical grade, extraction reagent for general medicinal; standard rutin (Rutin ,080 --- 9002) was purchased from Pharmaceutical and Biological Products. Experimental medicine purchase Fleabane White Church North County (2001-10) and identified as E. breviscapus Compositae Erigeronbreviscapus (Vant.) Hand. A Mazz. Seed. Youth Division cantharidin as coriander small yellow and black insects cantharidin, fn6cichoriiL. 2 Experimental methods and results of the standard curve, accurately weighed 2.1 rutin standard 11.90mg, with 60% ethanol solution, set the volume to 100ml, which was the standard solution concentration 0.119rag/ml. Standard reference Erigeron Injection l, measured the absorption of different concentrations of standard solution, data processing,[link widoczny dla zalogowanych], the regression equation A = 0.00462 +11.53 C (r = 0.9996, C: mg/m1). Standard solution, sample solution after the absorption of the color stability, 80 minutes,[link widoczny dla zalogowanych], standard measurement RSD = 0.170 (I1 = 9), sample determination RSD = 3.01 (n = 9). Full recovery was 97.1% ~ 102.8% (RSD = 4.133, n = 5). 2.2 Different Particle Size on the extraction of medicinal effects Table 1 of different solvents on the extraction of medicinal herbs to base Erigeron leather, leather base and cut into sections (about 1cm), the whole plant crushed (40 mesh) particle size for three compared. The same amount of water was added to the same extraction time, extraction of the same number of parallel test, extraction of total flavonoids were determined to medicine feeding amount into account, respectively, 4.71%, 4.70%,[link widoczny dla zalogowanych], 5.40% . From the experimental results, due to weak for small fleabane herb, plant height of about 10 ~ 20cm, loose texture, herbs, extracted in full when feeding grass or cut into sections, there is no difference; crushed extraction rate increased by about 0.7 percentage points ( about 15% relative increase), but filtering difficult, therefore, to feed the whole plant is most reasonable? 2.3 water and different concentrations of ethanol on the extraction of chemical constituents in accordance Erigeron physical and chemical properties, combined with production experience, choice of water and different concentrations of ethanol as extraction solvent contrast experiments. Whole plant Erigeron refluxed 3 times, extracts of total flavonoids were determined, the percentage of discount medicines feeding amount, results in Table 1. By the results, the highest rate of 50% ethanol extract, while the lowest rate of 95% ethanol extraction,[link widoczny dla zalogowanych], with water as solvent, the solvent extraction rate of close to optimal (relative extraction rate of 88%). Compared with water and dilute alcohol, low cost, safety, chlorophyll make less, therefore, water as solvent is the best choice. 2.4 ethyl acetate extraction of total flavonoids transfer rate Erigeron reflux medicine extracted 3 times with water, combined extract, vacuum concentrated ethanol precipitation treatment, alcohol precipitation solution overnight, filtration, recovery of ethanol extract thick measured with the Chinese Folk Medicine Section 58 of total 2002 volume of 3.16% (the percentage of feeding amount converted into medicine.) Mixing powder with hot water extract to ethyl acetate 3 times,[link widoczny dla zalogowanych], measured in the extract and the residual liquid content of total flavonoids were 1.32% (partial extraction), 1.84% (residual part). From the experimental circumstances and results, transfer of ethyl acetate as extraction solvent is low, the effect is not very good. The reason is mainly glycoside Erigeron class composition, polarity, ethyl glycoside class can not be extracted (extraction rate 42%), formed in the process of medicine utilization of the To improve the extraction rate, you can add a certain amount of ethanol, ethyl acetate extract to adjust the very 『health, improve the transfer rate of extraction.
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