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 Posted: Sat 14:00, 05 Mar 2011 Post subject: scarpe nike 2011 China's spawned some of hepatitis |
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,[link widoczny dla zalogowanych]
China's spawned some of hepatitis virus genome cloning and sequence analysis of cDNA
Series analysis results,[link widoczny dla zalogowanych], we have obtained fragment length 274bp (including both sides of the primers), shorter than expected 101lbp 737bp. From the sequence analysis and comparison of the results shows that the length of the PCR products were shorter than expected probably due to PCR by using primers P6 and the expected position of the non-template matching pairs in the expected location of the template on a non-nucleoside 7 acid with the P6 3 end primers fully complementary T nucleotides. Therefore, primers P6 primers may still play the role (PCR reaction primers complementary to 3 end of good is very important.) From the 'produce in the PCR reaction shorter than expected length of PCR products. May be due to the length of reverse transcription product or template secondary structure is not enough dry matter P6 l do not worry about leaving the correct position with the corresponding complementary. I visit Japan next PC'R matter 8t Jing good complementarity with the importance of the template. PCR primers for future reference design. Although P6I material is expected position of the non-template matching pairs,[link widoczny dla zalogowanych], but the P2,[link widoczny dla zalogowanych], PB; l thing known among the HDV sequence and homologous sequences of the corresponding location. Shows that the cloned fragment by P2 and HDVcDNA the correct position of the chain is expected to complement and PO-3l material and non-desired location HDVcDNA chain resulting from the HDVcDNA complementary fragments,[link widoczny dla zalogowanych], instead of non-specific PCR product.
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