nlwxpearo
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 Posted: Thu 0:04, 10 Mar 2011 Post subject: Madder pigment in vitro antibacterial activity _32 |
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Madder pigment in vitro antibacterial activity
mg, adding Tween 80 solubilization, dissolved in 1ml distilled water, the drug concentration 40Ixg/ml. Preparation of the 7H9 culture medium after a good, packed in sterilized test tube (180mmx18mm), each tube 10ml. Tube is divided into six groups, zero, one, two, three, four, five groups of three, numbered 1,2,3 tube, zero set of non-drug control group,[link widoczny dla zalogowanych], will be configured 1mg / ml of bacterial suspension was diluted to 10 times 10.2mg/ml, straw imbibe sterile broth 0.1m1 were seeded in each tube, the tube inoculation was 10.3mg. In one, two, three, four, five drugs were added with good 10l, 20l, 40IXl, 80IXl, 160IXl, each concentration for 40ixg/ml, 80ixg / 'ml, 160Ixg/ml, 320Ixg/ml, 640Ixg/m1. Incubator at 37 ℃. 24h after the drug after taking control in each group 1, 0.1ml medium inoculated LJ medium slope evenly distributed in the medium,[link widoczny dla zalogowanych], each concentration of the liquid medium inoculated with five modified Luo s medium and LJ medium inoculated with a good set plane gracefully, until the surface of the dried bacterial suspension inoculation, and then standing in the test tube rack, a good inoculation of Mycobacterium tuberculosis, will be plugged nozzle at 37 ℃ incubator to develop 25-30d. After 48h, 2 tubes of each group to take action as above, 1 weeks after tube No. 3 in each group above operation. In the training period, before the 10d, 3 ~ 4d observation 1, 10d, the day 1 observation, to be in control of the colonies grow to medium slant 2 / 3,[link widoczny dla zalogowanych], may terminate the training, record the results. Results 1 3 tubes of each group of about 25d after inoculation, culture, control colonies grow to medium slant 2 / 3, terminate the training, record the results,[link widoczny dla zalogowanych], the results are as follows. 2 tubes of each group of about 25d after culture inoculation, control colonies grow to medium slant 2 / 3, terminate the training, record the results, the results are as follows. 3 tubes of each group of about 25d after culture inoculation, control colonies grow to medium slant 2 / 3, terminate the training, record the results, the results are as follows. The results show that the control group two three four five, when the drug concentration 40Ixg/ml, 80Ixg/ml, 160Ixg/ml, the drug 24h, 48h and 1 week after inoculation the growth medium are Mycobacterium tuberculosis, when the drug concentration 320Ixg/ml, 640g/ml, the drug 24h, 48h and 1 week after inoculation the growth medium had no tuberculosis, proved madder elements in the drug concentration 320Ixg/ml can inhibit the growth of Mycobacterium tuberculosis. 4 shows results of the discussions, extracted from the madder madder hormone (1.2 dihydroxyanthraquinone) Mycobacterium tuberculosis in vitro experiments. Significant antibacterial activity. When the concentration of 160mg/ml drugs that can inhibit the growth of Mycobacterium tuberculosis, and the action time with no obvious relationship between drugs, drug 24h, 48h and 1 week after inoculation of solid media had no growth of Mycobacterium tuberculosis. The specific mechanism remains to be further studied. Anti-tuberculosis activity of traditional Chinese medicine, mostly multi-or single herb flavor extracts, though to prove its antibacterial activity, but for some drugs in some ingredient composition or synergistic effect of Mycobacterium tuberculosis is not clear, antibacterial mechanism thus will not be able to carry out. Thus, while Chinese medicine has obvious role of Mycobacterium tuberculosis in tuberculosis today the formation of multiple drug resistance, especially in the current anti-tuberculosis drug treatment order under the impact of multi-drug resistant cases, to open up traditional Chinese medicine Study of anti-TB medicine is a broad prospect of infinite area, but because the general mechanism of anti-TB medicine is not clear,[link widoczny dla zalogowanych], serious constraints in the traditional Chinese medicine applied in the treatment of tuberculosis. Through a variety of traditional Chinese medicine in the isolation of single components to the monomer in vitro antibacterial activity of initial screening of anti-TB treatment to find out the real role to play in the composition, the mechanism of how the role of these ingredients, This needs further study. 5 Conclusion fully proved by the experimental medicine monomer madder madder pigment extracted significantly in vitro activity of Mycobacterium tuberculosis. TB treatment for future research lays the foundation.
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