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Ionizing radiation and endonuclease induced DNA double-strand breaks as a carcinogenic and teratogenic cell death induced by sudden loss of critical


Lotus lDNaseI of RE DNA and no protein can be cut when the non-Wei. RE can be replaced with chromosomal DNA binding protein, has been Morgan (1991 years) confirmed. CHO cells in metaphase by AluI ~ FtSau3AI treatment, does not produce the typical chromosomal aberrations, but in the next split skirts, but a large number of chromosomal aberrations, suggesting that even in the metaphase chromatin protein folding and packaging and it was with the, RE is also have access to DNA. There is no doubt, by the RE cells in the mitotic role, G of the resulting distortion is the result of enzyme activity. The experiment appears in the high distortion, can be inside and outside medicine. Radiology plastic boat with the first 璺 bandit look toward the mid-fission energy is not allocated to make more films for ¨ j RE chromatid and chromosome aberration rate structure is AluI treatment of CHO cells for research. Experiments used heat shock treatment can prevent the AluI close to DNA, this effect can gum acid accumulation in non-histone proteins. On the contrary, within in a hypertonic salt solution, AluI can shoot CHO cells and human blood lymphocytes in patients be more chromosomal aberrations. This is because the hypertonic saline solution to make chromatin protein and DNA Results table becomes loose, so that more of the recognition sequence is exposed,[link widoczny dla zalogowanych], which is conducive to the role of RE. RE recognition sequence and chromosome and chromatid aberration rate within the RE Gu is a very complex interaction. DNA stain off from the calculation of the frequency and RE recognition sites caused by the correlation between chromosomal aberrations can not be pre-resistance, and then by the comparative analysis shows that the correlation between them exists. Base substitutions within the DNA, the change in the recognition sites, will also affect the cleavage activity of RE. Sticky ends with the flat end of the DSBBamHI and EcoRI DNA can generate all four bases of the sticky ends, the two RE cells can I deal with text from the light emblem chromosomal aberrations. Wiaeger and Pre-ston (1988 years) were used to produce flat beams end and the fools of the end of the RE were folded on the score, the analysis considered the frequency of their cleavage sites showed that these two types of RE can produce the same degree of chromosome distortion or cut flat at the end more effective. DNA helicase with the law, pulsed-field gel electrophoresis for determination of RE by electroporation guide) ~ CHO cells caused by the DSB, these experiments also confirmed the above two types of RE can produce the same level of DSB endonuclease enzymes eucalyptus hills induced chromosomal aberrations in the nature of a 『h from ionizing radiation can be used as a model chromosome aberrations. DSB in the induction of chromosome aberrations in this paper, the chemical structure of various types of DSB was discussed that the DSB is caused by different cellular mechanisms of chromosome play a role. VII-based solid-ionizing radiation can cause mutations in hprt gene, tk gene mutation. hprt gene mutation in most of the genetic changes, such as missing. Na / KATP mutant gene for the enzyme is not caused by radiation. Singh ~ [tBryant (1991 years) by the electroporation method into CHOK1 Pvu Ⅱ cells,[link widoczny dla zalogowanych], causing mutations and RE tk gene dose (5 ~ 3O units / nil) are dependent. EcoRI preached less active. In the tk gene, Pvu Ⅱ has 4 sites,[link widoczny dla zalogowanych], EcoRI and 6 loci, the authors identify that the RE sequence is homogeneous, experimental results support the PvuI induced DSB ends flat due to viscous than the EcoRI end more effectively lead to beam tk gene mutations. Other experiments show that, Alulfl ~ I play the hprt gene mutation V79 cells, but not caused by Na / KATP gene mutation. These data suggest that mammalian cells appear in vitro hprt gene and tk gene mutation is closely related to its DSB damage. Na / KATP gene is different,[link widoczny dla zalogowanych], the gene mutation is base pair changes. VIII cancer cells in vitro caused by ionizing radiation in vitro cultured mammalian cells to cancer has been known, DNaseI by liposomes on the Syrian hamster embryo cells after treatment, the morphological changes of cells were inoculated into newborn hamsters can cause tumors, indicating that the DSB is the critical damage caused by cancer. IX Summary of ionizing radiation, DNaseI, and RE can i play cell death, chromosomal aberrations, hprt gene and gene mutations and carcinogenesis. There is no doubt,[link widoczny dla zalogowanych], DSB is caused by the causes of these changes, but it's unclear how DSB caused by different types of biological end points and whether DSB are the same, these are worthy of discussion. Mutagenesi ~ 1992.7 (1): 3 ~ 12 (English) Sun Yuanming translation notes in the quotations section five school]